Theses
2024
Truncation of an aromatic phenyltransferase for the production of cannabinoids in Saccharomyces cerevisiae
Bachelor thesis by Joshua Lembeck
Supervisor:
Erin Jordan
Einfluss von Transporterproteinen und Transkriptionsfaktoren auf den Export von Olivetolsäure in Saccharomyces cerevisiae
Bachelor thesis by Sinja Maria Behnisch
Supervisor:
Christina Schmidt
Heterologe Synthese und Charakterisierung der Cholesterol-22-Hydroxylase aus Veratrum californicum in Saccharomyces cerevisiae
Bachelor thesis by Annika Arndt
Supervisor:
Marco Aras
Engineering of Saccharomyces cerevisiae for shikimate and malonyl-CoA pathway-derived precursor supply
Dissertation by Oliver Schiwy
Supervisor:
Oliver Kayser
Development of an analytical method for the detection of biosynthetic metabolites of vitamin D3 in Saccharomyces cerevisiae
Master thesis by Jan Dominic Graw
Supervisor:
Marco Aras
Advancement of cannabinoid biosynthesis through omics analysis: Systems biology, genome mining with structural biology, and cannabinomics
Dissertation by Erin Jordan
Supervisor:
Oliver Kayser
2023
Towards the heterologous production of rare cannabinoids - Bioengineering studies and rational protein design
Dissertation by Fabian Johannes Thomas
Supervisor:
Oliver Kayser
Optimization of the heterologous production of cannabinoids in Saccharomyces cerevisiae
Bachelor thesis by Jennifer Katharina Jakob
Supervisor:
Christina Schmidt
Construction of a fusion protein to optimise olivetolic acid production in Saccharomyces cerevisiae
Bachelor thesis by Lars Reckert
Supervisor:
Saskia Spitzer
Assay development for the formation of cannabinoids in protoplasts
Bachelor thesis by Nina Sonnenschein
A comparison of growth in cannabinoid-fed and unfed Saccharomyces cerevisiae CNPK2-1C shake flask cultures
Bachelor thesis by Sara Füst
Supervisor:
Erin Jordan
Optimisation of olivetolic acid production by fusion proteins in Saccharomyces cerevisiae
Bachelor thesis by David Kempel
Supervisor:
Saskia Spitzer
Establishment of an alternative quantification system for cannabigerolic acid production
Master thesis by Michelle Donner
Supervisor:
Saskia Spitzer
Expression of plant CBGAS in Saccharomyces cerevisiae and investigations on substrate specificity
Master thesis by Philipp Zellien
2022
Evaluation of plant callus cultures to elucidate the metabolic degradation of pesticides in plants
Dissertation by Leonie Hillebrands
Supervisor:
Oliver Kayser
Diversity and beneficial mechanisms of endophytic maytansine in Gymnosporia heterophylla defense and studies on biological activities of 20-hydroxymaytenine and related natural products
Dissertation by Thanet Pitakbut
Supervisor:
Oliver Kayser
Functional analysis of plant prenyltransferases in Saccharomyces cerevisiae
Bachelor thesis by Makram Fataeri
Supervisor:
Sophie Thomsen-Schmidt
Investigation of the hydrolytic activity of glucosidases from Saccharomyces cerevisiae towards cannabinoid glycosides
Master thesis by Astrid Imann
Supervisor:
Christina Schmidt
Exploration of alternate catalysts for drug development towards anti-cancer activity
Master thesis by Daniel Oliver Klin
2021
Quantification of geranyl pyrophosphate in mevalonate pathway-optimised strains of Saccharomyces cerevisiae
Bachelor thesis by Frederike Heinen
Supervisor:
Marco Aras
Heterologous expression of a UDP-glycosyltransferase from Cantharanthus roseus in Komagataella phaffii
Bachelor thesis by Robin Zimmer
Supervisor:
Christina Schmidt
Evaluation of the efficiency of selected IRES sequences in Saccharomyces cerevisiae
Bachelor thesis by Mara Loddenkemper
Supervisor:
Saskia Spitzer
Investigation of the structure-function relationships of THCA synthase with a focus on pH-dependent activity
Bachelor thesis by Melissa Konomi
Supervisor:
Fabian Thomas
The development of a fluorescence-based screening system for the detection of heterologously produced cannabinoid synthases in Saccharomyces cerevisiae
Bachelor thesis by Leon Frühauf
Supervisor:
Marco Aras
2020
Genomic integration of the genes ARO4K229L, ARO7G141S, and AroL in Saccharomyces cerevisiae to optimise the shikimate pathway
Bachelor thesis by Jonas Korb
Supervisor:
Oliver Schiwy
Construction of a fusion protein for the development of a screening system to optimise thcas expression in Saccharomyces cerevisiae
Bachelor thesis by Lina Schibajew
Supervisor:
Christina Schmidt
Structure-function relationships of cannabichromenoic acid synthase from Cannabis sativa
Bachelor thesis by Jan Baumeister
Supervisor:
Fabian Thomas
Investigation of different carboxylic acids as alternative substrates for cannabinoid biosynthesis in Saccharomyces cerevisiae
Bachelor thesis by Sarah Hallek
Supervisor:
Saskia Spitzer
Establishment of a testing system for quantification of geranyl pyrophosphate in metabolic engineered Saccharomyces cerevisiae
Master thesis by Aileen Schmidt
Supervisor:
Felix Stehle
Mevalonate pathway optimization to enhance GPP production by metabolic engineering in Saccharomyces cerevisiae
Master thesis by Janina K. Dahmen
Supervisor:
Marco Aras
2019
Molecular biological elucidation of late tropane alkaloid biosynthesis
Dissertation by Kathrin Laura Kohnen-Johannsen
Supervisor:
Oliver Kayser
Transcriptomic analysis of Radula marginata Taylor for identification of key genes in the biosynthesis of cannabinoid-like compounds
Dissertation by Tajammul Hussain
Supervisor:
Oliver Kayser
Establishment and development of methods for genetic modifications in plants and their effect on the metabolism
Dissertation by Julia Schachtsiek
Supervisors:
Oliver Kayser,
Felix Stehle
Etablierung eines Mediendurchsatzscreeningsystems zur Untersuchung von NphB-Varianten
Bachelor thesis by Sara Gröls
Supervisor:
Felix Stehle
Creation of a recombinant Saccharomyces cerevisiae strain to increase the rate of geranyl diphosphate synthesis
Bachelor thesis by Leonard Engels
Supervisor:
Felix Stehle
Investigations on the applicability of the virus-induced gene silencing system in Cannabis sativa L. using the cabbage leaf curl virus
Bachelor thesis by Leon Justen
Supervisor:
Julia Schachtsiek
Studies on the prevention of hyperhydricity in in vitro cell cultures of Cannabis sativa L.
Bachelor thesis by Alina Faye Weltersbach
Supervisor:
Julia Schachtsiek
Characterisation of the DeltaYPL062W deletion in Saccharomyces cerevisiae
Bachelor thesis by Joel Hünewinckell
Supervisor:
Christina Schmidt
Establishment of a BioLector-based medium-throughput screening system for the evaluation of GFP-tagged protein variants in yeast
Master thesis by Magdalena Thoma
Supervisor:
Felix Stehle
Alternative acyl-CoA synthetases for increased olivetol supply for cannbinoid synthesis in Saccharomycs cerevisiae
Master thesis by Lukas Greiwe
Supervisor:
Felix Stehle
In silico design of NphB variants to optimize the cannabigerolic acid production and characterization of promising variants in Saccharomyces cerevisiae
Master thesis by Saskia Spitzer
Supervisor:
Felix Stehle
Establishment of an HPLC-based method for the quantitative determination of eleven different cannabinoids as well as their isolation and purification
Master thesis by Benjamin Ryabenky
Supervisor:
Felix Stehle
2018
Recombinant expresson and functional characterization of cannabinoid producing enzymes in Komagataella phaffii
Dissertation by Bastian Zirpel
Supervisor:
Oliver Kayser
Evaluation of c-prenylating enzymes for the heterologous biosynthesis of cannabigerolic acid
Dissertation by Sara Friederike Degenhardt
Supervisor:
Oliver Kayser
Phytochemical and biological investigation of Jatropha pelargoniifolia roots native to the Kingdom of Saudi Arabia
Dissertation by Hanan Y. Aati
Supervisor:
Oliver Kayser
Cloning of the Hac1 gene to increase THCAS expression in S. cerevisiae
Bachelor thesis by David Bieniek
Supervisor:
Felix Stehle
Identification of common airborne microorganisms
Bachelor thesis by Dominik R. Bleidorn
Supervisor:
Armin Quentmeier
CRISPR/CAS9-based integration of olivetol synthase from Cannabis sativa L. into Saccharomyces cerevisiae
Bachelor thesis by Stefan Panzlaff
Supervisor:
Felix Stehle
Influence of growth conditions on hexanoic acid biosynthesis in Saccharomyces cerevisiae
Bachelor thesis by Katharina Tholen
Supervisor:
Felix Stehle
CRISPR/CAS9 based integration of HAC1s into the genome of Saccharomyces cerevisiae
Bachelor thesis by Thomas Robitzky
Supervisor:
Felix Stehle
CRISPR/CAS9-based knockout of the membrane transporter AQR1 to increase the intracellular hexanoic acid content in Saccharomyces cerevisiae
Bachelor thesis by Niklas Grimm
Supervisor:
Felix Stehle
Development of a transformation protocol for Cannabis sativa L. using Agrobacterium tumefaciens
Bachelor thesis by Ann-Katrin Rekendt
Implementation of a medium-chain fatty acid detection method for the optimization of cultivation conditions to improve the hexanoic acid biosynthesis in Saccharomyces cerevisiae
Master thesis by Hannah Wies
Supervisor:
Felix Stehle
Construction of different plasmids for the integration of olivetolic acid cyclase and olivetolic synthase into the yeast genome via DI-CRISPR/Cas9
Master thesis by Jonas Heyden
Supervisor:
Felix Stehle
Detection of human volatile metabolites with stationary and optimized non-stationary gas chromatography-ion mobility spectrometers
Master thesis by Igor Bergen
Supervisors:
Wolfgang Vautz,
Sascha Liedtke
Medium-throughput micro cultivation in yeast and crystallization of the prenyltransferase NphB
Master thesis by Tim-Marius Wunderlich
Supervisor:
Felix Stehle
Cannabis regeneration out of callus cultures
Master thesis by Ines de Ribeiro e Barreto Ferreira
Supervisor:
Julia Schachtsiek
Optimization of Delta9-tetrahydrocannabinolic acid synthase activity in Saccharomyces cerevisiae
Master thesis by Christina Schmidt
Supervisor:
Felix Stehle
2017
Identification, isolation and functional characterization of prenyltransferases in Cannabis sativa L.
Dissertation by Kathleen Pamplanyil
Supervisor:
Oliver Kayser
Optimisation of the heterologous expression of enterokinase in Escherichia coli
Bachelor thesis by Philip Konwicka
Supervisor:
Felix Stehle
Optimisation of stem-loop PCR for the detection of Cannabis sativa specific micro RNAs
Bachelor thesis by Katharina Kaufmann
Supervisor:
Felix Stehle
Cloning and characterisation of the cannabinoid metabolism in E. coli for the heterologous production of canabigerolic acid
Bachelor thesis by Christoph-Alexander Schrage
Influence of different signal peptides on the intracellular localisation of THCA synthase in Saccharomyces cerevisiae
Bachelor thesis by Tim Orthwein
Supervisor:
Felix Stehle
Expression studies of NphB using different vector systems
Bachelor thesis by Sarah Dybowski
Supervisor:
Felix Stehle
CRISPR-Cas9 mediated pathway design for an improved precursor supply for heterologous cannabinoid production in Saccharomyces cerevisiae
Master thesis by Hanna Schnittger
Supervisor:
Felix Stehle
Design and characterization of NphB variants for an optimized cannbigerolic acid production
Master thesis by Martin Lukas Kares
Supervisor:
Felix Stehle
Detection of Cannabis sativa and other illicit drug compounds with stationary and non-stationary gas chromatography-ion mobility spectrometers
Master thesis by Christoph Stoll
Supervisors:
Wolfgang Vautz,
Sascha Liedtke
Development of a cultivation and downstream process for high yield production of NphB for protein crystallization
Master thesis by Dirk Münker
Supervisor:
Felix Stehle
Design of an automated low throughput screening platform for the optimization of Delta9-tetrahydrocannabinolic acid production in Komagataella phaffii
Master thesis by Florian Worm
Supervisor:
Felix Stehle
2016
Towards a platform organism for terpenoid production - in silico analysis of metabolic networks of potential hosts and in vivo validation
Dissertation by Evamaria Gruchattka
Supervisor:
Oliver Kayser
Influence of abiotic factors on growth and biosynthesis of secondary plant components in Duboisia species
Dissertation by Sophie Friederike Ullrich
Supervisor:
Oliver Kayser
The influence of phytohormones and miRNAs on trichome development in Cannabis sativa
Bachelor thesis by Laura-Marie Zimmermann
Supervisor:
Felix Stehle
Strategies for the targeted isolation and enrichment of cannabinoids from Cannabis sativa
Bachelor thesis by Lisa Milena Bischoff
Supervisor:
Armin Quentmeier
Heterologous expression and functional characterization of olivetolic acid cyclase and olivetol synthase for olivetolic acid production
Master thesis by Tobias Gräf
Supervisor:
Felix Stehle
Optimization and scale up of the heterologous expression of NphB in Escherichia coli
Master thesis by Daniel Stalinski
Supervisor:
Felix Stehle
CRISPR-Cas9 mediated genome-editing of Saccharomyces cerevisiae
Master thesis by Nico Biermann
Supervisor:
Felix Stehle
Studies on the uptake of olivetol and olivetolic acid by Saccharomyces cerevisiae
Bachelor thesis by Juliana Estrela
2015
Creation and characterisation of NphB variants with regard to substrate specificity
Bachelor thesis by Ramona Hansmeyer
Supervisor:
Felix Stehle
Purification of delta9-tetrahydrocannabinol acid synthase (THCA synthase) from Cannabis sativa
Bachelor thesis by Christoph Stoll
Supervisor:
Armin Quentmeier
Heterologous expression and purification of delta9-tetrahydrocannabinolic acid synthase
Bachelor thesis by Anna Heitkamp
Supervisor:
Armin Quentmeier
Investigation of limiting factors of delta9-tetrahydrocannabinolic acid synthase expression in Saccharomyces cerevisiae
Bachelor thesis by Martin Obholzer
Supervisor:
Felix Stehle
Extraction and purification of cannabinoids from varieties of Cannabis sativa
Bachelor thesis by Matusah Gnaneswaran
Supervisor:
Armin Quentmeier
Synthesis and characterisation of geranyl diphosphate (GPP) for cannabinoid biosynthesis
Bachelor thesis by Julia Grühn
Supervisor:
Felix Stehle
Mitochondrial targeting of heterologously expressed THCAS in Saccharomyces cerevisiae
Master thesis by Soeren Alsleben
Supervisor:
Felix Stehle
Optimization of recombinant human cytochrome expression in E. coli for in vitro conversion of THC metabolites
Master thesis by Katrin Stych
Supervisor:
Felix Stehle
Expression and purification of recombinant ß-1,3-glucanase
Master thesis by Christian Staubach
Supervisor:
Felix Stehle
Protein engineering of a soluble prenyltransferase: Design and characterization of NphB towards altered substrate specifity
Master thesis by Marlon Wach
Supervisor:
Felix Stehle
2014
Cannabinoid production in Cannabis sativa L.: An in vitro approach
Dissertation by Sayed Farag
Supervisor:
Oliver Kayser
Isolation, identification and characterization of endophytes from Cannabis sativa L. and Radula marginata
Dissertation by Parijat Kusari
Supervisor:
Oliver Kayser
Metabolomics, proteomics, and transcriptomics of Cannabis sativa L. trichomes
Dissertation by Nizar Happyana
Supervisor:
Oliver Kayser
Extraction, isolation and identification of cannabinoids from Cannabis sativa L.
Bachelor thesis by Helene Sertznig
Supervisor:
Armin Quentmeier
Heterologous expression of Delta9-Tetrahydrocannabinololic acid synthase in Saccharomyces cerevisiae
Bachelor thesis by David Dannheisig
Supervisor:
Felix Stehle
Heterologous expression of delta9-tetrahydrocannabinolic acid synthase in strains of Saccharomyces cerevisiae
Bachelor thesis by Jana Rogoschin
Supervisor:
Armin Quentmeier
Heterologous expression of delta9-tetrahydrocannabinolic acid synthase in Escherichia coli
Bachelor thesis by Madeleine Dorsch
Supervisor:
Felix Stehle
Optimisation of protein purification of delta9-tetrahydrocannabinolic acid synthase by affinity chromatography
Bachelor thesis by Anna Engelke
Supervisor:
Felix Stehle
Heterologous expression of NphB in Escherichia coli
Master thesis by Christine Finger
Supervisor:
Felix Stehle
Metabolic engineering of Saccharomyces cerevisiae for patchoulol production
Master thesis by Nicole Jurisch
Supervisor:
Evamaria Gruchattka
Study of the composition of the essential oil and trichome fluid of Cannabis sativa and proteomic profiling of cannabinoid biosynthesis in the trichomes
Master thesis by Andrzej Nowicki
2013
The cannabigerolic acid synthase (CBGAS) from Cannabis sativa L. - Studies on heterologous expression in Saccharomyces cerevisiae
Bachelor thesis by Susan Henning
Supervisor:
Felix Stehle
Isolation of tetrahydrocannabinolic acid synthase from Saccharomyces cerevisae YPL154C
Bachelor thesis by Katharina Becker
Supervisor:
Armin Quentmeier
Heterologous expression of tetrahydrocannabinolic acid synthase in Pichia pastoris
Bachelor thesis by Sebastian Kollenda
Supervisor:
Armin Quentmeier
Heterologous expression of delta9-tetrahydrocannabinolic acid synthase in Saccharomyces cerevisiae
Bachelor thesis by Dirk Münker
Supervisor:
Felix Stehle
Synthetic biology for the production of tetrahydrocannabinolic acid (THCA) from Cannabis sativa in Saccharomyces cerevisiae
Master thesis by Sara Friederike Degenhardt
Supervisor:
Verena Schütz
Metabolic engineering of Saccharomyces cerevisiae for monoterpenoid production
Master thesis by Katharina Smolczyk
Supervisors:
Verena Schütz,
Felix Stehle
2012
Cryopreservation, chemical and biochemical stability in hairy root cultures of Cannabis sativa var. bedrobinol
Bachelor thesis by Johanna Petranowitsch
Supervisor:
Sayed Farag
Expression of Delta1-Tetrahydrocannabinolic acid synthase in Saccharomyces cerevisiae
Master thesis by Joanna Hajduk
Patchoulol production in Saccharomyces cerevisiae - Metabolic Engineering and process optimization
Master thesis by Michael Felten
2011
Production of tetrahydrocannabinol metabolites with human recombinant P450 - Investigation of analytical, enzyme kinetic and process engineering requirements
Bachelor thesis by Michael Felten
Supervisor:
Torsten Arndt
Expression of Hyoscyamine-6ß-Hydroxylase in genetically modified E. coli and S. cerevisiae for the conversion of Hyoscyamine to Scopolamine
Diploma thesis by Nils Averesch
Supervisor:
Marcello Siena
Production of scopolamine by Saccharomyces cerevisiae
Master thesis by Elzbieta Budziak
Search for cannabinoids-producing microbes among the endophytes of Cannabis sativa
Master thesis by Dominika Neumann
Production of monoterpenoids in the heterologous host Saccharomyces cerevisiae
Master thesis by Jacqueline Wolf
Supervisor:
Remco Muntendam
Characterization of aromatic prenyltransferases from higher plants and microbial origins
Master thesis by Katja Straub