Theses
PhD Theses
| Titel | Name | Datum |
| Plant-based aromatic prenyltransferases for activation of phenolic compounds | Dr. Sophie Thomsen-Schmidt | July 2023 |
| Towards the heterologous production of rare cannabinoids - Bioengineering studies and rational design | Dr. Fabian Johannes Thomas | May 2023 |
| Diversity and beneficial mechanisms of endophytic maytansine in Gymnosporia heterophylla defense and studies on biological activities of 20-hydroxymaytenine and related natural products | Dr. Thanet Pitakbut | October 2022 |
| Evaluation of plant callus cultures to elucidate the metabolic degradation of pesticides in plants | Dr. Leonie Hillebrands | January 2022 |
| Establishment and development of methods for genetic modifications in plants and their effect on the metabolism | Dr. Julia Schachtsiek | October 2019 |
| Transcriptomic analysis of Radula marginata Taylor for identification of key genes in the biosynthesis of cannabinoid-like compounds | Dr. Tajammul Hussain | June 2019 |
| Molecular biological elucidation of late tropane alkaloid biosynthesis | Dr. Kathrin Laura Kohnen-Johannsen | June 2019 |
| Phytochemical and biological investigation of Jatropha pelargoniifolia roots native to the Kingdom of Saudi Arabia | Dr. Hanan Y. Aati | December 2018 |
| Evaluation of c-prenylating enzymes for the heterologous biosynthesis of cannabigerolic acid | Dr. Sara Friederike Degenhardt | October 2018 |
| Recombinant expression and functional characterization of cannabinoid producing enzmes in Komagataella phaffii | Dr. Bastian Zirpel | May 2018 |
| Identification, isolation and functional characterization of prenyltransferases in Cannabis sativa L. | Dr. Kathleen Pamplanyil | March 2017 |
| Influence of abiotic factors on growth and biosynthesis of secondary plant components in Duboisia species | Dr. Sophie Friederike Ullrich | December 2016 |
| Towards a platform organism for terpenoid production - in silico analysis of metabolic networks of potential hosts and in vivo validation | Dr. Evamaria Gruchattka | May 2016 |
| Metabolomics, proteomics, and transcriptomics of Cannabis sativa L. trichomes | Dr. Nizar Happyana | December 2014 |
| Isolation, identification and characterization of endophytes from Cannabis sativa L. and Radula marginata | Dr. Parijat Kusari | December 2014 |
| Cannabinoid production in Cannabis sativa L.: An in vitro approach | Dr. Sayed Farag | November 2014 |
| Xanthophyllomyces dendrorhous as a platform organism for the production of terpenes | Dr. Elena Melillo | March 2013 |
| Endophytes as alternative paclitaxel sources: chemistry and genetics of Taxomyces andreanae and the endophytic flora of Wollenia mobilis | Dr. Agata Staniak | 2010 |
| Identification, characterization and expression of early biosynthesis genes from Artemisia annua for the heterologous biosynthesis of dihydroartemisinic acid as a first step towards artemisinin production | Dr. Anna-Margareta Rydén | 2010 |
| Studies of Antrhiscus sylvestris L. (Hoffm.) - Metabolic engineering of combinatorial biosynthesis of podophyllotoxin | Dr. Oktavia Hendrawati | 2008 |
| New formulation approaches for the leishmanicidal drug Miltefosine and for the polyene antibiotic Amphotericin B | Dr. Andreas Lemke | 2008 |
Diploma and Master theses
Diploma Thesis
| Titel Expression of Hyoscyamine-6ß-Hydroxylase in genetically modified E. coli and S. cerevisiae for the conversion of Hyoscyamine to Scopolamine | Name Nils Averesch | Date December 2011 |
Master Theses
| Titel | Name | Date |
| Expression of plant CBGAS in Saccharomyces cerevisiae and investigations on substrate specificity | Philipp Zellien | June 2023 |
| Establishment of an alternative quantification system for cannabigerolic acid production | Michelle Donner | March 2023 |
| Exploration of alternate catalysts for drug development towards anti-cancer activity | Daniel Oliver Klin | July 2022 |
| Investigation of the hydrolytic activity of glucosidases from Saccharomyces cerevisiae towards cannabinoid glycosides | Astrid Imann | June 2022 |
| Mevalonate pathway optimization to enhance GPP production by metabolic engineering in Saccharomyces cerevisiae | Janina K. Dahmen | December 2020 |
| Establishment of a testing system for quantification of geranyl pyrophosphate in metabolic engineered Saccharomyces cerevisiae | Aileen Schmidt | January 2020 |
| Establishment of an HPLC-based method for the quantitative determination of eleven different cannabinoids as well as their isolation and purification | Benjamin Ryabenky | November 2019 |
| In silico design of NphB variants to optimize the cannabigerolic acid production and characterization of promising variants in Saccharomyces cerevisiae | Saskia Spitzer | August 2019 |
| Alternative acyl-CoA synthetases for increased olivetol supply for cannabinoid synthesis in Saccharomyces cerevisiae | Lukas Greiwe | June 2019 |
| Establishment of a BioLector-based medium-throughput screening system for the evaluation of GFP-tagged protein variants in yeast | Magdalena Thoma | June 2019 |
| Optimization of Delta9-tetrahydrocannabinolic acid synthase activity in Saccharomyces cerevisiae | Christina Schmidt | December 2018 |
| Cannabis regeneration out of callus cultures | Ines de Ribeiro e Barreto Ferreira | November 2018 |
| Medium-throughput micro cultivation in yeast and crystallization of the prenyltransferase NphB | Tim-Marius Wunderlich | September 2018 |
| Detection of human volatile metabolites with stationary and optimized non-stationary gas chromatography-ion mobility spectrometers | Igor Bergen | April 2018 |
| Construction of different plasmids for the integration of olivetolic acid cyclase and olivetolic synthase into the yeast genome via DI-CRISPR/Cas9 | Jonas Heyden | January 2018 |
| Implementation of a medium-chain fatty acid detection method for the optimization of cultivation conditions to improve the hexanoic acid biosynthesis in Saccharomyces cerevisiae | Hannah Wies | January 2018 |
| Design of an automated low throughput screening platform for the optimization of Delta9-tetrahydrocannabinolic acid production in Komagataella phaffii | Florian Worm | December 2017 |
| Development of a cultivation and downstream process for high yield production of NphB for protein crystallization | Dirk Münker | December 2017 |
| Detection of Cannabis sativa and other illicit drug compounds with stationary and non-stationary gas chromatography-ion mobility spectrometers | Christoph Stoll | November 2017 |
| Design and characterization of NphB variants for an optimized cannbigerolic acid production | Martin Lukas Kares | July 2017 |
| CRISPR-Cas9 mediated pathway design for an improved precursor supply for heterologous cannabinoid production in Saccharomyces cerevisiae | Hanna Schnittger | June 2017 |
| Studies on the uptake of olivetol and olivetolic acid by Saccharomyces cerevisiae | Juliana Estrela | December 2016 |
| CRISPR-Cas9 mediated genome-editing of Saccharomyces cerevisiae | Nico Biermann | August 2016 |
| Optimization and scale up of the heterologous expression of NphB in Escherichia coli | Daniel Stalinski | August 2016 |
| Heterologous expression and functional characterization of olivetolic acid cyclase and olivetol synthase for olivetolic acid production | Tobias Gräf | August 2016 |
| Protein engineering of a soluble prenyltransferase: Design and characterization of NphB towards altered substrate specifity | Marlon Wach | July 2015 |
| Expression and purification of recombinant ß-1,3-glucanase | Christian Staubach | June 2015 |
| Optimization of recombinant human cytochrome expression in E. coli for in vitro conversion of THC metabolites | Katrin Stych | March 2015 |
| Mitochondrial targeting of heterologously expressed THCAS in Saccharomyces cerevisiae | Soeren Alsleben | March 2015 |
| Study of the composition of the essential oil and trichome fluid of Cannabis sativa and proteomic profiling of cannabinoid biosynthesis in the trichomes | Andrzej Nowicki | 2014 |
| Metabolic engineering of Saccharomyces cerevisiae for patchoulol production | Nicole Jurisch | November 2014 |
| Heterologous expression of NphB in Escherichia coli | Christine Finger | July 2014 |
| Metabolic engineering of Saccharomyces cerevisiae for monoterpenoid production | Katharina Smolczyk | July 2013 |
| Synthetic biology for the production of tetrahydrocannabinolic acid (THCA) from Cannabis sativa in Saccharomyces cerevisiae | Sara Friederike Degenhardt | May 2013 |
| Patchoulol production in Saccharomyces cerevisiae - Metabolic Engineering and process optimization | Michael Felten | October 2012 |
| Expression of Delta1-Tetrahydrocannabinolic acid synthase in Saccharomyces cerevisiae | Joanna Hajduk | September 2012 |
| Characterization of aromatic prenyltransferases from higher plants and microbial origins | Katja Straub | November 2011 |
| Production of monoterpenoids in the heterologous host Saccharomyces cerevisiae | Jacqueline Wolf | November 2011 |
| Search for cannabinoids-producing microbes among the endophytes of Cannabis sativa | Dominika Neumann | June 2011 |
| Production of scopolamine by Saccharomyces cerevisiae | Elzbieta Budziak | June 2011 |
| The use of site-directed mutagenesis in probing the acitve site of amorpha-4,11-diene synthase | Marta Ilecke | 2010 |
| Fungal endophytes and their natural products | Krysztof Przybylowski | 2008 |
| Bioanalytical characterisation of wound healing constituents from Lucilia sericata maggots | Karol Gustav Czaja | 2008 |
| Taxomyces andreanae, enigmatic producer of taxol, demystified. Detection of taxandes in the endophytes of yew | Katarzyna Krawczyk | 2007 |
| Development of an HPLC-UV method for the determination of scopoletin and umckalin in Pelargonium sidoides extracts and its commercial products | Marta Piestrzynska | 2006 |
| Detection of taxadiene sythase gene in Taxomyces andreanae by means of PCR | Magdalena Czepnik | 2006 |
| Genetic based screening for imvproved natural product discovery in Arabidopsis thaliana | Agata Staniak | 2004 |
| Isolation, structure elucidation and determination of immunomodulatory activity of Pelargonium sidoides extract Eps 7630 and its constituents | Weronika Trun | 2004 |
Bachelor Theses
| Titel | Name | Date |
| A comparison of growth in cannabinoid-fed and unfed Saccharomyces cerevisiae CENPK2-1c shake flask cultures | Sara Füst | February 2023 |
| Assay Entwicklung für die Bildung von Cannabinoiden in Protoplasten | Nina Sonnenschein | February 2023 |
| Konstruktion eines Fusionsproteins zur Optimierung der Olivetolsäureproduktion in Saccharomyces cerevisiae | Lars Reckert | February 2023 |
| Optimization of the heterologous production of cannabinoids in Saccharomyces cerevisiae | Jennifer Katharina Jakob | January 2023 |
| Functional analysis of plant prenyltransferases in Saccharomyces cerevisiae | Makram Fataeri | August 2022 |
Die Entwicklung eines fluoreszenbasierten Screeningsystems zum Nachweis heterolog produzierter Cannabinoidsynthasen in Saccharomyces cerevisiae | Leon Frühauf | October 2021 |
| Untersuchung der Struktur-Funktionsbeziehungen der THCA-Synthase mit Fokus auf pH-abhängige Aktivität | Melissa Konomi | September 2021 |
| Evaluierung der Effizienz ausgewählter IRES-Sequenzen in Saccharomyces cerevisiae | Mara Loddenkemper | May 2021 |
| Heterologe Expression einer UDP-Glykosyltransferase aus Cantharanthus roseus in Komagataella phaffii | Robin Zimmer | April 2021 |
| Quantifizierung von Geranylpyrophosphat in Mevalonatweg-optimierten Stämmen von Saccharomyces cerevisiae | Frederike Heinen | January 2021 |
| Untersuchung von verschiedenen Carbonsäuren als alternative Substrate der Cannabinoidbiosynthese in Saccharomyces cerevisiae | Sarah Hallek | December 2020 |
| Struktur-Funktionsbeziehungen der Cannabichromensäuresynthase aus Cannabis sativa | Jan Baumeister | October 2020 |
| Konstruktion eines Fusionsproteins für die Entwicklung eines Screeningsystems zur Optimierung der thcas Expression in Saccharomyces cerevisiae | Lina Schibajew | October 2020 |
| Genomische Integration der Gene ARO4K229L, ARO7G141S, und AroL in Saccharomyces cerevisiae zur Optimierung des Shikimatwegs | Jonas Korb | February 2020 |
| Charakterisierung der Deletion DeltaYPL062W in Saccharomyces cerevisiae | Joel Hünewinckell | December 2019 |
| Untersuchungen zur Vermeidung von Hyperhydrizität in in vitro Zellkulturen von Cannabis sativa L. | Alina Faye Weltersbach | November 2019 |
| Untersuchungen zur Anwendbarkeit des Virus-induzierten Gen-Silencing-Systems in Cannabis sativa L. unter Verwendung des cabbage leaf curl virus | Leon Justen | October 2019 |
| Erstellung eines rekombinanten Saccharomyces cerevisiae-Stammes zur Steigerung der Geranyldiphosphatsyntheserate | Leonard Engels | April 2019 |
| Etablierung eines Mediendurchsatzscreeningsystems zur Untersuchung von NphB-Varianten | Sara Gröls | April 2019 |
| Ausarbeitung eines Transformationsprotokolls für Cannabis sativa L. mittels Agrobacterium tumefaciens | Ann-Katrin Rekendt | September 2018 |
| CRISPR/CAS9-basierter Knockout des Membrantransporters AQR1 zur Steigerung des intrazellulären Hexansäuregehaltes in Saccharomyces cerevisiae | Niklas Grimm | September 2018 |
| CRISPR/CAS9 basierte Integration von HAC1s in das Genom von Saccharomyces cerevisiae | Thomas Robitzky | July 2018 |
| Einfluss der Wachstumsbedingungen auf die Hexansäurebiosynthese in Saccharomyces cerevisiae | Katharina Tholen | June 2018 |
| CRISPR/CAS9-basierte Integration der Olivetolsynthase aus Cannabis sativa L. in Saccharomyces cerevisiae | Stefan Panzlaff | April 2018 |
| Identifikation häufiger Luftkeime | Dominik R. Bleidorn | April 2018 |
| Klonierung des Hac1-Gens zur Steigerung der THCAS-Expression in S. cerevisiae | David Bieniek | March 2018 |
| Expressionsstudien von NphB unter Verwendung verschiedener Vektorsysteme | Sarah Dybowski | August 2017 |
| Einfluss verschiedener Signalpeptide auf die intrazelluläre Lokalisierung der THCA-Synthse in Saccharomyces cerevisiae | Tim Orthwein | August 2017 |
| Klonierung und Charakterisierung des Cannabinoidstoffwechsels in E. coli zur heterologen Produktion von Canabigerolsäure | Christoph Alexander Schrage | June 2017 |
| Optimierung der Stem-loop PCR zur Detektion Cannabis sativa spezifischer micro RNAs | Katharina Kaufmann | May 2017 |
| Optimierung der heterologen Expression von Enterokinase in Escherichia coli | Philip Konwicka | May 2017 |
| Strategien zur gezielten Isolierung und Anreicherung von Cannabinoiden aus Cannabis sativa | Lisa Milena Bischoff | November 2016 |
| Der Einfluss von Phytohormonen und miRNAs auf die Trichomentwicklung in Cannabis sativa | Laura-Marie Zimmermann | September 2016 |
| Synthese und Charakterisierung von Geranyldiphosphat (GPP) für die Cannabinoidbiosynthese | Julia Grühn | October 2015 |
| Extraktion und Reinigung von Cannabinoiden aus Varietäten von Cannabis sativa | Matusah Gnaneswaran | October 2015 |
| Untersuchung von Limitierungsfaktoren der Delta9-Tetrahydrocannabinolsäure-Synthase-Expression in Saccharomyces cerevisiae | Martin Obholzer | September 2015 |
| Heterologe Expression und Aufreinigung von Delta9-Tetrahydrocannabinolsäure-Synthase | Anna Heitkamp | September 2015 |
| Anreicherung von Delta9-Tetrahydrocannabinolsäure-Synthase (THCA-Synthase) aus Cannabis sativa | Christoph Stoll | July 2015 |
| Erstellung und Charakterisierung von NphB-Varianten hinsichtlich der Substratspezifität | Ramona Hansmeyer | March 2015 |
| Optimierung der Proteinaufreinigung der Delta9-Tetrahydrocannabinolsäure-Synthase mittels Affinitätschromatographie | Anna Engelke | September 2014 |
| Heterologe Expression der Delta9-Tetrahydrocannabinolsäure-Synthase in Escherichia coli | Madeleine Dorsch | September 2014 |
| Heterologe Expression der Delta9-Tetrahydrocannabinolsäure-Synthase in Stämmen von Saccharomyces cerevisiae | Jana Rogoschin | September 2014 |
| Heterologe Expression der Delta9-Tetrahydrocannabinolsäure-Synthase in Saccharomyces cerevisiae | David Dannheisig | April 2014 |
| Extraktion, Isolierung und Identifikation von Cannabinoiden aus Cannabis sativa L. | Helene Sertznig | April 2014 |
| Heterologe Expression der Delta9-Tetrahydrocannabinolsäure-Synthase in Saccharomyces cerevisiae | Dirk Münker | December 2013 |
| Heterologe Expression von Tetrahydrocannabinolsäure-Synthase in Pichia pastoris | Sebastian Kollenda | October 2013 |
| Isolierung von Tetrahydrocannabinolsäure-Sythase aus Saccharomyces cerevisae YPL154C | Katharina Becker | February 2013 |
| Die Cannabigerolsäure-Synthase (CBGAS) aus Cannabis sativa L. -Untersuchungen zur heterologen Expression in Saccharomyces cerevisiae | Susan Henning | February 2013 |
| Cryopreservation, chemical and biochemical stability in hairy root cultures of Cannabis sativa var. bedrobinol | Johanna Petranowitsch | September 2012 |
| Herstellung von Tetrahydrocannabinol-Metaboliten mit humanem, rekombinanten P450 - Untersuchung analytischer, enzymkinetischer und verfahrenstechnischer Voraussetzungen | Michael Felten | March 2011 |