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Faculty of Biochemical and Chemical engineering


PhD Theses

Titel Name Datum
Plant-based aromatic prenyltransferases for activation of phenolic compounds Dr. Sophie Thomsen-Schmidt July 2023
Towards the heterologous production of rare cannabinoids - Bioengineering studies and rational design Dr. Fabian Johannes Thomas May 2023
Diversity and beneficial mechanisms of endophytic maytansine in Gymnosporia heterophylla defense and studies on biological activities of 20-hydroxymaytenine and related natural products Dr. Thanet Pitakbut October 2022
Evaluation of plant callus cultures to elucidate the metabolic degradation of pesticides in plants Dr. Leonie Hillebrands January 2022
Establishment and development of methods for genetic modifications in plants and their effect on the metabolism Dr. Julia Schachtsiek October 2019
Transcriptomic analysis of Radula marginata Taylor for identification of key genes in the biosynthesis of cannabinoid-like compounds Dr. Tajammul Hussain June 2019
Molecular biological elucidation of late tropane alkaloid biosynthesis Dr. Kathrin Laura Kohnen-Johannsen June 2019
Phytochemical and biological investigation of Jatropha pelargoniifolia roots native to the Kingdom of Saudi Arabia Dr. Hanan Y. Aati December 2018
Evaluation of c-prenylating enzymes for the heterologous biosynthesis of cannabigerolic acid Dr. Sara Friederike Degenhardt October 2018
Recombinant expression and functional characterization of cannabinoid producing enzmes in Komagataella phaffii Dr. Bastian Zirpel May 2018
Identification, isolation and functional characterization of prenyltransferases in Cannabis sativa L. Dr. Kathleen Pamplanyil March 2017
Influence of abiotic factors on growth and biosynthesis of secondary plant components in Duboisia species Dr. Sophie Friederike Ullrich December 2016
Towards a platform organism for terpenoid production - in silico analysis of metabolic networks of potential hosts and in vivo validation Dr. Evamaria Gruchattka May 2016
Metabolomics, proteomics, and transcriptomics of Cannabis sativa L. trichomes Dr. Nizar Happyana December 2014
Isolation, identification and characterization of endophytes from Cannabis sativa L. and Radula marginata Dr. Parijat Kusari December 2014
Cannabinoid production in Cannabis sativa L.: An in vitro approach Dr. Sayed Farag November 2014
Xanthophyllomyces dendrorhous as a platform organism for the production of terpenes Dr. Elena Melillo March 2013
Endophytes as alternative paclitaxel sources: chemistry and genetics of Taxomyces andreanae and the endophytic flora of Wollenia mobilis Dr. Agata Staniak 2010
Identification, characterization and expression of early biosynthesis genes from Artemisia annua for the heterologous biosynthesis of dihydroartemisinic acid as a first step towards artemisinin production Dr. Anna-Margareta Rydén 2010
Studies of Antrhiscus sylvestris L. (Hoffm.) - Metabolic engineering of combinatorial biosynthesis of podophyllotoxin Dr. Oktavia Hendrawati 2008
New formulation approaches for the leishmanicidal drug Miltefosine and for the polyene antibiotic Amphotericin B Dr. Andreas Lemke 2008

Diploma and Master theses

Diploma Thesis


Expression of Hyoscyamine-6ß-Hydroxylase in genetically modified E. coli and S. cerevisiae for the conversion of Hyoscyamine to Scopolamine

Nils Averesch

December 2011

Master Theses

Titel Name Date
Expression of plant CBGAS in Saccharomyces cerevisiae and investigations on substrate specificity Philipp Zellien June 2023
Establishment of an alternative quantification system for cannabigerolic acid production Michelle Donner March 2023
Exploration of alternate catalysts for drug development towards anti-cancer activity Daniel Oliver Klin July 2022
Investigation of the hydrolytic activity of glucosidases from Saccharomyces cerevisiae towards cannabinoid glycosides Astrid Imann June 2022
Mevalonate pathway optimization to enhance GPP production by metabolic engineering in Saccharomyces cerevisiae Janina K. Dahmen December 2020
Establishment of a testing system for quantification of geranyl pyrophosphate in metabolic engineered Saccharomyces cerevisiae Aileen Schmidt January 2020
Establishment of an HPLC-based method for the quantitative determination of eleven different cannabinoids as well as their isolation and purification Benjamin Ryabenky November 2019
In silico design of NphB variants to optimize the cannabigerolic acid production and characterization of promising variants in Saccharomyces cerevisiae Saskia Spitzer August 2019
Alternative acyl-CoA synthetases for increased olivetol supply for cannabinoid synthesis in Saccharomyces cerevisiae Lukas Greiwe June 2019
Establishment of a BioLector-based medium-throughput screening system for the evaluation of GFP-tagged protein variants in yeast Magdalena Thoma June 2019
Optimization of Delta9-tetrahydrocannabinolic acid synthase activity in Saccharomyces cerevisiae Christina Schmidt December 2018
Cannabis regeneration out of callus cultures Ines de Ribeiro e Barreto Ferreira November 2018
Medium-throughput micro cultivation in yeast and crystallization of the prenyltransferase NphB Tim-Marius Wunderlich September 2018
Detection of human volatile metabolites with stationary and optimized non-stationary gas chromatography-ion mobility spectrometers Igor Bergen April 2018
Construction of different plasmids for the integration of olivetolic acid cyclase and olivetolic synthase into the yeast genome via DI-CRISPR/Cas9 Jonas Heyden January 2018
Implementation of a medium-chain fatty acid detection method for the optimization of cultivation conditions to improve the hexanoic acid biosynthesis in Saccharomyces cerevisiae Hannah Wies January 2018
Design of an automated low throughput screening platform for the optimization of Delta9-tetrahydrocannabinolic acid production in Komagataella phaffii Florian Worm December 2017
Development of a cultivation and downstream process for high yield production of NphB for protein crystallization Dirk Münker December 2017
Detection of Cannabis sativa and other illicit drug compounds with stationary and non-stationary gas chromatography-ion mobility spectrometers Christoph Stoll November 2017
Design and characterization of NphB variants for an optimized cannbigerolic acid production Martin Lukas Kares July 2017
CRISPR-Cas9 mediated pathway design for an improved precursor supply for heterologous cannabinoid production in Saccharomyces cerevisiae Hanna Schnittger June 2017
Studies on the uptake of olivetol and olivetolic acid by Saccharomyces cerevisiae Juliana Estrela December 2016
CRISPR-Cas9 mediated genome-editing of Saccharomyces cerevisiae Nico Biermann August 2016
Optimization and scale up of the heterologous expression of NphB in Escherichia coli Daniel Stalinski August 2016
Heterologous expression and functional characterization of olivetolic acid cyclase and olivetol synthase for olivetolic acid production Tobias Gräf August 2016
Protein engineering of a soluble prenyltransferase: Design and characterization of NphB towards altered substrate specifity Marlon Wach July 2015
Expression and purification of recombinant ß-1,3-glucanase Christian Staubach June 2015
Optimization of recombinant human cytochrome expression in E. coli for in vitro conversion of THC metabolites Katrin Stych March 2015
Mitochondrial targeting of heterologously expressed THCAS in Saccharomyces cerevisiae Soeren Alsleben March 2015
Study of the composition of the essential oil and trichome fluid of Cannabis sativa and proteomic profiling of cannabinoid biosynthesis in the trichomes Andrzej Nowicki 2014
Metabolic engineering of Saccharomyces cerevisiae for patchoulol production Nicole Jurisch November 2014
Heterologous expression of NphB in Escherichia coli Christine Finger July 2014
Metabolic engineering of Saccharomyces cerevisiae for monoterpenoid production Katharina Smolczyk July 2013
Synthetic biology for the production of tetrahydrocannabinolic acid (THCA) from Cannabis sativa in Saccharomyces cerevisiae Sara Friederike Degenhardt May 2013
Patchoulol production in Saccharomyces cerevisiae - Metabolic Engineering and process optimization Michael Felten October 2012
Expression of Delta1-Tetrahydrocannabinolic acid synthase in Saccharomyces cerevisiae Joanna Hajduk September 2012
Characterization of aromatic prenyltransferases from higher plants and microbial origins Katja Straub November 2011
Production of monoterpenoids in the heterologous host Saccharomyces cerevisiae Jacqueline Wolf November 2011
Search for cannabinoids-producing microbes among the endophytes of Cannabis sativa Dominika Neumann June 2011
Production of scopolamine by Saccharomyces cerevisiae Elzbieta Budziak June 2011
The use of site-directed mutagenesis in probing the acitve site of amorpha-4,11-diene synthase Marta Ilecke 2010
Fungal endophytes and their natural products Krysztof Przybylowski 2008
Bioanalytical characterisation of wound healing constituents from Lucilia sericata maggots Karol Gustav Czaja 2008
Taxomyces andreanae, enigmatic producer of taxol, demystified. Detection of taxandes in the endophytes of yew Katarzyna Krawczyk 2007
Development of an HPLC-UV method for the determination of scopoletin and umckalin in Pelargonium sidoides extracts and its commercial products Marta Piestrzynska 2006
Detection of taxadiene sythase gene in Taxomyces andreanae by means of PCR Magdalena Czepnik 2006
Genetic based screening for imvproved natural product discovery in Arabidopsis thaliana Agata Staniak 2004
Isolation, structure elucidation and determination of immunomodulatory activity of Pelargonium sidoides extract Eps 7630 and its constituents Weronika Trun 2004

Bachelor Theses

Titel Name Date
A comparison of growth in cannabinoid-fed and unfed Saccharomyces cerevisiae CENPK2-1c shake flask cultures Sara Füst February 2023
Assay Entwicklung für die Bildung von Cannabinoiden in Protoplasten Nina Sonnenschein February 2023
Konstruktion eines Fusionsproteins zur Optimierung der Olivetolsäureproduktion in Saccharomyces cerevisiae Lars Reckert February 2023
Optimization of the heterologous production of cannabinoids in Saccharomyces cerevisiae Jennifer Katharina Jakob January 2023
Functional analysis of plant prenyltransferases in Saccharomyces cerevisiae Makram Fataeri August 2022

Die Entwicklung eines fluoreszenbasierten Screeningsystems zum Nachweis heterolog produzierter Cannabinoidsynthasen in Saccharomyces cerevisiae

Leon Frühauf

October 2021
Untersuchung der Struktur-Funktionsbeziehungen der THCA-Synthase mit Fokus auf pH-abhängige Aktivität Melissa Konomi September 2021
Evaluierung der Effizienz ausgewählter IRES-Sequenzen in Saccharomyces cerevisiae Mara Loddenkemper May 2021
Heterologe Expression einer UDP-Glykosyltransferase aus Cantharanthus roseus in Komagataella phaffii Robin Zimmer April 2021
Quantifizierung von Geranylpyrophosphat in Mevalonatweg-optimierten Stämmen von Saccharomyces cerevisiae Frederike Heinen January 2021
Untersuchung von verschiedenen Carbonsäuren als alternative Substrate der Cannabinoidbiosynthese in Saccharomyces cerevisiae Sarah Hallek December 2020
Struktur-Funktionsbeziehungen der Cannabichromensäuresynthase aus Cannabis sativa Jan Baumeister October 2020
Konstruktion eines Fusionsproteins für die Entwicklung eines Screeningsystems zur Optimierung der thcas Expression in Saccharomyces cerevisiae Lina Schibajew October 2020
Genomische Integration der Gene ARO4K229L, ARO7G141S, und AroL in Saccharomyces cerevisiae zur Optimierung des Shikimatwegs Jonas Korb February 2020
Charakterisierung der Deletion DeltaYPL062W in Saccharomyces cerevisiae Joel Hünewinckell December 2019
Untersuchungen zur Vermeidung von Hyperhydrizität in in vitro Zellkulturen von Cannabis sativa L. Alina Faye Weltersbach November 2019
Untersuchungen zur Anwendbarkeit des Virus-induzierten Gen-Silencing-Systems in Cannabis sativa L. unter Verwendung des cabbage leaf curl virus Leon Justen October 2019
Erstellung eines rekombinanten Saccharomyces cerevisiae-Stammes zur Steigerung der Geranyldiphosphatsyntheserate Leonard Engels April 2019
Etablierung eines Mediendurchsatzscreeningsystems zur Untersuchung von NphB-Varianten Sara Gröls April 2019
Ausarbeitung eines Transformationsprotokolls für Cannabis sativa L. mittels Agrobacterium tumefaciens Ann-Katrin Rekendt September 2018
CRISPR/CAS9-basierter Knockout des Membrantransporters AQR1 zur Steigerung des intrazellulären Hexansäuregehaltes in Saccharomyces cerevisiae Niklas Grimm September 2018
CRISPR/CAS9 basierte Integration von HAC1s in das Genom von Saccharomyces cerevisiae Thomas Robitzky July 2018
Einfluss der Wachstumsbedingungen auf die Hexansäurebiosynthese in Saccharomyces cerevisiae Katharina Tholen June 2018
CRISPR/CAS9-basierte Integration der Olivetolsynthase aus Cannabis sativa L. in Saccharomyces cerevisiae Stefan Panzlaff April 2018
Identifikation häufiger Luftkeime Dominik R. Bleidorn April 2018
Klonierung des Hac1-Gens zur Steigerung der THCAS-Expression in S. cerevisiae David Bieniek March 2018
Expressionsstudien von NphB unter Verwendung verschiedener Vektorsysteme Sarah Dybowski August 2017
Einfluss verschiedener Signalpeptide auf die intrazelluläre Lokalisierung der THCA-Synthse in Saccharomyces cerevisiae Tim Orthwein August 2017
Klonierung und Charakterisierung des Cannabinoidstoffwechsels in E. coli zur heterologen Produktion von Canabigerolsäure Christoph Alexander Schrage June 2017
Optimierung der Stem-loop PCR zur Detektion Cannabis sativa spezifischer micro RNAs Katharina Kaufmann May 2017
Optimierung der heterologen Expression von Enterokinase in Escherichia coli Philip Konwicka May 2017
Strategien zur gezielten Isolierung und Anreicherung von Cannabinoiden aus Cannabis sativa Lisa Milena Bischoff November 2016
Der Einfluss von Phytohormonen und miRNAs auf die Trichomentwicklung in Cannabis sativa Laura-Marie Zimmermann September 2016
Synthese und Charakterisierung von Geranyldiphosphat (GPP) für die Cannabinoidbiosynthese Julia Grühn October 2015
Extraktion und Reinigung von Cannabinoiden aus Varietäten von Cannabis sativa Matusah Gnaneswaran October 2015
Untersuchung von Limitierungsfaktoren der Delta9-Tetrahydrocannabinolsäure-Synthase-Expression in Saccharomyces cerevisiae Martin Obholzer September 2015
Heterologe Expression und Aufreinigung von Delta9-Tetrahydrocannabinolsäure-Synthase Anna Heitkamp September 2015
Anreicherung von Delta9-Tetrahydrocannabinolsäure-Synthase (THCA-Synthase) aus Cannabis sativa Christoph Stoll July 2015
Erstellung und Charakterisierung von NphB-Varianten hinsichtlich der Substratspezifität Ramona Hansmeyer March 2015
Optimierung der Proteinaufreinigung der Delta9-Tetrahydrocannabinolsäure-Synthase mittels Affinitätschromatographie Anna Engelke September 2014
Heterologe Expression der Delta9-Tetrahydrocannabinolsäure-Synthase in Escherichia coli Madeleine Dorsch September 2014
Heterologe Expression der Delta9-Tetrahydrocannabinolsäure-Synthase in Stämmen von Saccharomyces cerevisiae Jana Rogoschin September 2014
Heterologe Expression der Delta9-Tetrahydrocannabinolsäure-Synthase in Saccharomyces cerevisiae David Dannheisig April 2014
Extraktion, Isolierung und Identifikation von Cannabinoiden aus Cannabis sativa L. Helene Sertznig April 2014
Heterologe Expression der Delta9-Tetrahydrocannabinolsäure-Synthase in Saccharomyces cerevisiae Dirk Münker December 2013
Heterologe Expression von Tetrahydrocannabinolsäure-Synthase in Pichia pastoris Sebastian Kollenda October 2013
Isolierung von Tetrahydrocannabinolsäure-Sythase aus Saccharomyces cerevisae YPL154C Katharina Becker February 2013
Die Cannabigerolsäure-Synthase (CBGAS) aus Cannabis sativa L. -Untersuchungen zur heterologen Expression in Saccharomyces cerevisiae Susan Henning February 2013
Cryopreservation, chemical and biochemical stability in hairy root cultures of Cannabis sativa var. bedrobinol Johanna Petranowitsch September 2012
Herstellung von Tetrahydrocannabinol-Metaboliten mit humanem, rekombinanten P450 - Untersuchung analytischer, enzymkinetischer und verfahrenstechnischer Voraussetzungen Michael Felten March 2011